Synergistic effects of the inoculation with Pseudomonas putida, Rhizobium legominosarum bv. phaseoli and Glomus mosseae on the performance of Common Bean (Phaseolus vulgaris L.)

Abstract
In order to investigate the effects of the inoculation with P. putida, R. leguminosarum bv. phaseoli and F. mosseae on yield and nutrient uptake of Common Bean (Phaseolus vulgaris L.), a greenhouse experiment in a completely randomized factorial design with 3 treatment including a: microorganism in four level (no inoculation, P. putida, F. mosseae and mix inoculation of fungi and bacteria), B: Zn in two level (0 and 10 mg.kg-1) and C: Nitrogen in two leve (inoculation with R. leguminosarum and adding 70 mg.kg-1 nitrogen in form of NH4NO3) with four replication was conducted. The results of experiment indicated that the concentration of P, K, Zn, Cu and Mn in shoots of plants and shoot dry matter, shoot wet matter, seed dry weight, seed number and thousand seed weight affected by simultaneous inoculation of F. mosseae and P. putida. The concurrent inoculation of F. mosseae + P. putida + R. leguminosarum bv. phaseoli significantly increased the Mn concentration in shoots of plants and seed number. Uptake of N, P, K, Fe, Zn, Cu and Mn significantly affected by synchronous inoculation of F. mosseae and P. putida. The concurrent inoculation of F. mosseae + P. putida + R. leguminosarum bv. Phaseoli had no significant effect on nutrient uptake. Maximum thousand seed weight was obtained in rhizobium treatment which represents the role of effective and efficient rhizobium bacteria in reduction of nitrogen fertilizers use without yield loss.
Keywords: Iron, Seed number, Zinc, Copper, Manganese, Growth indices.
Abstract
In order to investigate the effects of the inoculation with P. putida, R. leguminosarum bv. phaseoli and F. mosseae on yield and nutrient uptake of Common Bean (Phaseolus vulgaris L.), a greenhouse experiment in a completely randomized factorial design with 3 treatment including a: microorganism in four level (no inoculation, P. putida, F. mosseae and mix inoculation of fungi and bacteria), B: Zn in two level (0 and 10 mg.kg-1) and C: Nitrogen in two leve (inoculation with R. leguminosarum and adding 70 mg.kg-1 nitrogen in form of NH4NO3) with four replication was conducted. The results of experiment indicated that the concentration of P, K, Zn, Cu and Mn in shoots of plants and shoot dry matter, shoot wet matter, seed dry weight, seed number and thousand seed weight affected by simultaneous inoculation of F. mosseae and P. putida. The concurrent inoculation of F. mosseae + P. putida + R. leguminosarum bv. phaseoli significantly increased the Mn concentration in shoots of plants and seed number. Uptake of N, P, K, Fe, Zn, Cu and Mn significantly affected by synchronous inoculation of F. mosseae and P. putida. The concurrent inoculation of F. mosseae + P. putida + R. leguminosarum bv. Phaseoli had no significant effect on nutrient uptake. Maximum thousand seed weight was obtained in rhizobium treatment which represents the role of effective and efficient rhizobium bacteria in reduction of nitrogen fertilizers use without yield loss.
Keywords: Iron, Seed number, Zinc, Copper, Manganese, Growth indices.