Evaluation of effect of salinity stress and foliar spraying of spermine, spermidine and putrescine on leaf and root nutrients in Camelina

Document Type : Complete scientific research article

Authors

1 Associate professor, Department of Agricultural Sciences, Payame Noor University, Tehran, Iran

2 Assistant professor, Department of Agricultural Sciences, Payame Noor University, Tehran, Iran

Abstract

Background and Objectives: Soil salinity, especially in arid and semi-arid regions, is one of the factors that limit the growth of plants. Excessive accumulation of sodium and chloride ions leads to reduced growth and imbalance of nutrients. The use of exogenous polyamines, including putrescine, spermidine and spermine, is considered as an effective method not only in determining their role in response to salinity, but also as a way to increase plant resistance to salinity. Camelina is an oilseed product of the Brassica family, which contains other bioactive compounds such as flavonoids and phenolic products and as a new source of essential fatty acids, especially omega-3 fatty acids. This study was conducted with the aim of evaluating the effect of different levels of sodium chloride and foliar spraying of spermine, spermidine and putrescine on seed yield and the concentration of leaf and root nutrients in Camelina plant.
Materials and methods: The experiment was carried out in March 2022 as factorial design based on a completely random design (CRD) in the form of pot culture with 12 treatments and 3 replications. The salinity stress treatment was with Urmia lake water at three levels (0, 15, 30 ds/m). Foliar treatment at 4 levels 1- Foliar spraying with spermine (2 mM), 2- Foliar spraying with spermidine (2 mM), 3- Foliar spraying with putrescine (2 mM) and control (no foliar spraying). The application time of foliar spraying was done every 3 days (totally in 6 stages) after establishing three seedlings in the pot and reaching the four-leaf stage. At the end of the Camelina plant growth season, to calculate the seed yield, the seeds in the capsules of three plants in each pot were separated and weighed. Statistical analysis of data was done using SAS software (version 9.1) and MATATC, and comparison of means was also done by Tukey's test at the five percent level. Excel program was also used to draw graphs.
Results: The results of this research showed that the salt stress was 30 and 15 ds/m compared to the treatment without salt decreased leaf potassium (52 and 17%), root potassium (44 and 37%), leaf magnesium (50 and 28%), root magnesium (56 and 24%), leaf zinc (58 and 47%), root zinc (39 and 29%), leaf iron (10 and 2%), root iron (57 and 23%) and seed yield (52 and 10%), but it increased the amount of leaf sodium (89 and 82%), root sodium (39 and 11%), leaf calcium (14 and 6%) and root calcium (76 and 28%), respectively. Also, salinity stress caused a decrease in the ratio of potassium to sodium absorption, but increased the ratio of calcium to the sum of sodium and potassium in the roots and leaves due to the increase in the amount of sodium and the decrease in potassium. At all salinity levels, foliar spraying with polyamines increased leaf and root potassium, leaf and root magnesium, leaf and root zinc, leaf and root iron, by reducing the amount of sodium in leaves and roots. Also, foliar spraying with spermine, spermidine and putrescine compared to no foliar spraying, increased the seed yield by 32, 8 and 21%, respectively.

Conclusion: The results of this research showed that foliar spraying with spermine, spermidine and putrescine by improving the absorption of macro and micro elements in roots and leaves, increasing the ratio of potassium to sodium and the ratio of calcium to total sodium and potassium in roots and leaves could moderate the effects of salinity stress and improve the seed yield of camellia under salinity stress conditions and it prevented excessive reduction in seed yield.

Keywords

Main Subjects

References

1.FAO. (2008). FAO Land and Plant Nutrition Management Service.
2.Fariduddin, Q., Mir, B. A., Yusuf, M., & Ahmad, A. (2013). Comparative roles of brassinosteroids and polyamines in salt stress tolerance. Acta Physiologiae Plantarum, 35(7), 2037-2053.
3.Diao, Q., Song, Y., & Qi, H. (2015). Exogenous spermidine enhances chilling tolerance of tomato (Solanum lycopersicum L.) seedlings via involvement in polyamines metabolism and physiological parameter levels. Acta Physiologiae Plantarum, 37(11), 230.
4.Sharma, A., Slathia, S., Pal, S., Sharma, Y., & Langer, A. (2014). Role of 24-Epibrassinolide, Putrescine and Spermine in Salinity Stressed Adiantum capillus-veneris Leaves. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 84, 183-192.
5.Acosta-Motos, J. R., Ortuño, M. F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M. J., & Hernandez,
J. A. (2017). Plant Responses to Salt Stress: Adaptive Mechanisms. Agronomy, 7(1), 1-38.
6.Sun, J., Chen, S. L., Dai, S. X., Wang,R. G., Li, N. Y., Shen, X., Zhou, X. Y., Lu, C. F., Zheng, X. J., Hu, Z. M., Zhang, Z. K., Song, J., & Xu, Y. (2009). Ion flux profiles and plant ion homeostasis control under salt stress. Plant Signaling & Behavior, 4, 261-4.
7.Yu, Z., Duan, X., Luo, L., Dai, S., Ding, Z., & Xia, G. (2020). How Plant Hormones Mediate Salt Stress Responses. Trends in Plant Science, 25(11), 1117-1130.
8.Balal, R. M., Shahid, M. A., Khan, N., Sarkhosh, A., Zubair, M., Rasool, A., Mattson, N., Gomez C., Bukhari, M. A., Waleed, M., & Nasim, W. (2022). Morphological, Physiological, and Biochemical Modulations in Crops under Salt Stress, in Building Climate Resilience in Agriculture, W.N.J. et al., Editor, Springer International Publishing.
9.Min, W., Guo, H., Zhou, G., Zhang, W., Ma, L., Ye, J., & Hou, Z. (2014). Root distribution and growth of cotton as affected by drip irrigation with saline water. Field Crops Research, 169, 1-10.
10.Wakeel, A. (2013). Potassium–sodium interactions in soil and plant under saline-sodic conditions. Journal of
Plant Nutrition and Soil Science, 176(3), 344-354.
11.Hassanpouraghdam, M. B., Mehrabani, L. V., & Tzortzakis, N. (2020). Foliar application of nano-zinc and iron affects physiological attributes of rosmarinus officinalis and quietens nacl salinity depression. Journal of Soil Science and Plant Nutrition, 20(2), 335-345.
12.Novo, L. A., Covelo, E. F., & González, L. (2014). Effect of salinity on zinc uptake by Brassica juncea. International Journal of Phytoremediation, 16(12-7), 704-718.
13.Tolay, I. (2021). The impact of different Zinc (Zn) levels on growth and nutrient uptake of Basil (Ocimum basilicum L.) grown under salinity stress. PLOS ONE, 16, e0246493.
14.Alghamdi, E., Farooq, M., & Metwali, E. (2022). Influence of nano zinc oxide on the in vitro callus growth, ex vitro tuber yield and nutritional quality of potato (Solanum tuberosum L.) cultivars under salt stress. Journal of Animal and Plant Sciences, 32, 440-449.
15.Mirvat, E. G., Mohamed, M. H., & Tawfik, M. M. (2006). Effect of phosphorus fertilizer and foliar spraying with zinc on growth, yield and quality of groundnut under reclaimed sandy soils. Journal of Applied Science Research, 2(8), 491-496.
16.Boostani, H. R., & Farrokhnejad, E. (2018). Effect of plant growth promoting rhizobacteria, mycorrhizae fungi and salinity stress on the uptake of some nutrients by corn (Zea mays L.). Journal of Plant Process and Function, 7(24), 39-52.
17.Roychoudhury, A., Basu, S., & Sengupta, D. N. (2011). Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology, 168(4), 317-28.
18.Radhakrishnan, R., & Lee, I. J. (2013). Regulation of salicylic acid, jasmonic acid and fatty acids in cucumber (Cucumis sativus L.) by spermidine promotes plant growth against salt stress. Acta Physiologiae Plantarum, 35(12), 3315-3322.
19.Chattopadhayay, M. K., Tiwari, B. S., Chattopadhyay, G., Bose, A., Sengupta, D. N., & Ghosh, B. (2002). Protective role of exogenous polyamines on salinity-stressed rice (Oryza sativa) plants. Physiology and Plant, 116(2), 192-199.
20.Hussein, M., Nadia, E., Gereadly, H., & El-Desuki, M. (2006). Role of putrescine in resistance to salinity of pea plants (Pisum sativum L.). Journal of Applied Sciences Research, 2(9), 598-604.
21.Baniasadi, F., Saffari, V. R., & Maghsoudi Moud, A. A. (2018). Physiological and growth responses of Calendula officinalis L. plants to the interaction effects of polyamines and salt stress. Scientia Horticulturae, 234, 312-317.
22.Ibrahim, F., & El Habbasha, E. S. (2015). Chemical composition, medicinal impacts and cultivation of camelina (Camelina sativa): Review. 8, 114-122.
23.Angelini, L. G., Abou Chehade, L., Foschi, L., & Tavarini, S. (2020). Performance and potentiality of camelina (Camelina sativa L. crantz) genotypes in response to sowing date under mediterranean environment. Agronomy, 10(12), 1929.
24.Keshavarz, H. (2020). Study of water deficit conditions and beneficial microbes on the oil quality and agronomic traits of canola (Brassica napus L.). Grasas y Aceites, 71(3), e373.
25.Heydarian, Z., Yu, M., Gruber, M., Coutu, C., Robinson, S. J., & Hegedus, D. D. (2018). Changes in gene expression in Camelina sativa roots and vegetative tissues in response to salinity stress. Scientific Reports, 8(1), 9804.
26.Kazemi, S., Rafati Alashti, M., & Hosseini, S. J. (2022). Response of Biochemical and Physiological Properties of camellia (Camelina sativa L.) to Foliar Application of Calcium and Silicon Nanoparticles. Silicon, 14(12), 6817-6828.
27.Alizadeh, A. (2000). The relationship between water, soil and plants. Mashhad: Astan Quds Publications. Emam RezaUniv, 616p. (In Persian)
28.William, H. (2000). Official methods of analysis of AOAC international. 17nd ed. Association Official Analytical Chemists. USA. 100.
29.Gholamian, S. M., Ghamarnia, H., & Kahrizy, D. (2017). Effects of saline water on Camelina (Camelina sativa) yield in Greenhouse condition.Water and Irrigation Management, 7(2), 333-348.
30.Kaya, C., Ashraf, M., Alyemeni, M. N., & Ahmad, P. (2020). The role of endogenous nitric oxide in salicylic acid-induced up-regulation of ascorbate-glutathione cycle involved in salinity tolerance of pepper (Capsicum annuum L.) plants. Plant Physiology and Biochemistry, 147, 10-20.
31.Talaat, N. B., Mahmoud, A. W. M., & Hanafy, A. M. A. (2022). Co-application of salicylic acid and spermine
alleviates salt stress toxicity in wheat: growth, nutrient acquisition, osmolytes ccumulation, and antioxidant response. Acta Physiologiae Plantarum, 45(1), 1.
32.Aelaei, M., Karami, Z., Arghavani, M., & Salehi, F. (2021). The Study of effects of spermine under salinity stress on orphophysiological characteristics of Catharanthus roseus L‎. Iranian Journal of Horticultural Science, 52(3), 553-564.
33.Zlatev, Z., & Yordanov, I. (2004). Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Journal of Plant Physiology, 30, 3-4.
34.Ashraf, M. (2001). Relationships between growth and gas exchange characteristics in some salt-tolerant amphidiploid Brassica species in relation to their diploid parents. Environmental and Experimental Botany, 45, 155-163.
35.Alamer, K. H., Perveen, S., Khaliq, A., Zia Ul Haq, M., Ibrahim, M. U., & Ijaz, B. (2022). Mitigation of salinity stress in maize seedlings by the application of vermicompost and sorghum water extracts. Plants, 11(19), 2548.
36.Rostampour, P., Hamidian, M., Dehnavi, M. M., & Saeidimajd, G. A. (2023). Evaluation of osmoregulation and morpho-physiological responses of Borago officinalis under drought and salinity stress with equal osmotic potential. Biochemical Systematics and Ecology, 106, 104567.
37.Abbas, M., Younis, M., & Shukry, W. (1991). Plant growth, metabolism and adaptation in relation to stress conditions. XIV. Effect of salinity on the internal solute concentrations in Phaseolus vulgaris. Journal of Plant Physiology, 138(6), 722-727.
38.Moradi, S., Jahanban, L., & Basaki, T. (2022). Concentration of micronutrient in Azola at cadmium and salinity contaminated environment. Human & Environment, 20(3), 217-230.
39.Bardel, J., Ghanbari, A., & Khajeh, M. (2016). Physiological response and polyamines content of cumin (Cuminum cyminum L.) to water irrigation quality in the application of chemical and organic fertilizers. Iranian Journal of Medicinal and Aromatic Plants Research, 32(2), 360-375.
40.Hussain, S., Shaukat, M., Ashraf, M., Zhu, C., Jin, Q., & Zhang, J. (2019). Salinity stress in arid and semi-arid climates: Effects and management in field crops. Climate Change and Agriculture, 13.
41.Hezaveh, T. A., Rahmani, F., Alipour, H., & Pourakbar, L. (2020). Effects of foliar application of ZnO nanoparticles on secondary metabolite and micro-elements of camelina (Camelina sativa L.) under salinity stress. Journal of Stress Physiology & Biochemistry, 16(4), 54-69.
42.Epstein, E. (1998). How Calcium Enhances Plant Salt Tolerance. Science, 280(5371), 1906-1907.
43.Turhan, E., & Eris, A. (2004). Effects of sodium chloride applications and different growth media on ionic composition in strawberry plant. Journal of Plant Nutrition, 27, 1653-1665.
44.Billard, V., Maillard, A., Coquet, L., Jouenne, T., Cruz, F., Garcia-Mina, J. M., Yvin, J. C., Ourry, A., & Etienne, P. (2016). Mg deficiency affects leaf Mg remobilization and the proteome in Brassica napus. Plant Physiology and Biochemistry, 107, 343-373.
45.Wolf, F. I., & Trapani, V. (2007). Cell (patho) physiology of magnesium. Clinical Science, 114(1), 27-35.
46.Miura, K. (2013). Nitrogen and Phosphorus Nutrition Under Salinity Stress, in Ecophysiology and Responses of Plants under Salt Stress, P. Ahmad, M.M. Azooz, and M.N.V. Prasad, Editors., Springer New York: New York, NY. 425-441.
47.Yildirim, E., Karlidag, H., & Turan, M. (2009). Mitigation of salt stress in strawberry by foliar K, Ca and Mg nutrient supply. Plant, Soil and Environment, 55, 213-221.
48.Momenpour, A., Bakhshi, D., Imani, A., & Rezaie, H. (2015). Effect of salinity stress on growth characteristics and concentrations of nutrition elements in almond ‘Shahrood 12’, ‘Touno’ cultivars and ‘1-16’ genotype budded on
GF677 rootstock. Journal of Crops Improvement, 17(1), 197-216.
49.Pashangeh, Z., Abdolahi, F., & Ghasemi, M. (2020). The interaction of salinity and gibberellin on leaf abscission, dry matter, antioxidant enzymes activity and ion content in guava (Psidium guajava L). Journal of Plant Research (Iranian Journal of Biology), 33(4), 809-826.
50.Askari, M., Amini, F., & Jamali, F. (2015). Effects of zinc on growth, photosynthetic pigments, proline, carbohydrate and protein content of Lycopersicum esculentum under salinity. Journal of Plant Process and Function, 3, 45-58.
51.Farahat, M. M., Ibrahim, M. M., Taha, L., & El-Quesni, E. M. (2007). Response of vegetative growth and some chemical constituents of Cupressus sempervirens L. to foliar application of ascorbic acid and zinc at Nubaria. World Journal of Agricultural Sciences, 3, 496-502.
52.Siddiqui, S. N., Umar, S., & Iqbal, M. (2015). Zinc-induced modulation of some biochemical parameters in a high- and a low-zinc-accumulating genotype of Cicer arietinum L. grown under Zn-deficient condition. Protoplasma, 252(5), 1335-1345.
53.Mateos‐Naranjo, E., Redondo‐Gómez, S., Cambrollé, J., Luque, T., & Figueroa, M. (2008). Growth and photosynthetic responses to zinc stress of an invasive cordgrass, Spartina densiflora. Plant Biology, 10(6), 754-762.
54.Munns, R., & Tester, M. (2008). Mechanisms of Salinity Tolerance. Annual Review of Plant Biology, 59(1), 651-681.
55.Nenova, V. (2008). Growth and mineral concentrations of pea plants under different salinity levels and iron supply. General and Applied Plant Physiology, 34(3-4), 189-202.
56.Iqbal, M., & Ashraf, M. (2005). Changes in growth, photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed treatment with polyamines. Plant Growth Regulation, 46(1), 19-30.
57.Kamiab, F., Talaie, A., Khezri, M., & Javanshah, A. (2014). Exogenous application of free polyamines enhance salt tolerance of pistachio (Pistacia vera L.) seedlings. Plant Growth Regulation, 72, 257-268.
58.Sajid Aqeel Ahmad, M., Javed, F., & Ashraf, M. (2007). Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissue of two indica rice (Oryza sativa L.) genotypes. Plant Growth Regulation, 53(1), 53-63.
59.Wu, Y., Hu, Y., & Xu, G. (2008). Interactive effects of potassium and sodium on root growth and expression of K/Na transporter genes in rice. Plant Growth Regulation, 57(3), 271-280.
60.Patel, P., Kajal, S., Patel, V., Patel, V., & Kristi, S. S. (2010). Impact of salt stress on nutrient uptake and growth of cowpea. Brazilian Journal of Plant Physiology, 22, 43-48.
61.Netondo, G., Onyango, J. C., & Beck, E. (2004). Sorghum and salinity: I. Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Science, 44, 797-805.
62.Ketehouli, T., Idrice Carther, K. F., Noman, M., Wang, F. W., Li, X. W., & Li, H. Y. (2019). Adaptation of plants to salt stress: Characterization of Na+ and K+ transporters and role of CBL gene family in regulating salt stress response. Agronomy, 9(11), 1-32.
63.Farsaraei, S., Mehdizadeh, L., & Moghaddam, M. (2021). Seed priming with putrescine alleviated salinity stress during germination and seedling growth of medicinal pumpkin. Journal of Soil Science and Plant Nutrition, 21(3), 1782-1792.
64.Raziq, A., Mohi Ud Din, A., Anwar, S., Wang, Y., Jahan, M. S., He, M., Ling, C. G., Sun, J., Shu, S., & Guo, S. (2022). Exogenous spermidine modulates polyamine metabolism and improves stress responsive mechanisms to protect tomato seedlings against salt stress. Plant Physiology and Biochemistry, 187, 1-10.
65.Iqbal, M., & Ashraf, M. (2010). Changes in hormonal balance: a possible mechanism of pre‐sowing chilling‐induced salt tolerance in spring wheat. Journal of Agronomy and Crop Science, 196(6), 440-454.
Journal of Soil Management and Sustainable Production
Volume 13, Issue 3
September 2023
Pages 1-24

Files

Share

How to cite

Statistics